李宝库 1,2柳乐 1,2徐伟 1曾文彬 1[ ... ]蔡盛 1,*
作者单位
摘要
1 中国科学院长春光学精密机械与物理研究所,吉林 长春 130033
2 中国科学院大学,北京 100049
探测距离是红外系统应用的重要评价指标,随着制冷型红外探测器的发展,红外系统自身热辐射已成为探测距离提升的重要限制性因素,冷光学设计是抑制自身热辐射的必然选择,因此对冷光学制冷温度指标进行评估和优化成为红外系统设计分析的新问题。文中从红外系统自身热辐射和经典探测距离理论出发,推导了包含系统噪声项的红外系统探测距离计算公式,提出了分布式探测距离的分析方法。以透射式光学系统为例,进行了影响因素灵敏度分析。通过对探测器焦平面进行分区域数据处理,得到了对应探测距离的主要影响表面。在此基础上,分析了在对主要影响表面进行低温处理前后(293.15 K制冷到173.15 K)探测距离的变化。结果表明,探测距离最大提升量达到43.32%,提升效果显著。该方法可为红外系统冷光学设计和评估提供参考。
红外探测系统 自身热辐射 探测距离 主要影响表面 分布式变化 infrared detection system self-thermal radiation detection range main influence surface distributed change 
红外与激光工程
2023, 52(3): 20220417
刘乐 1,2廖旻晶 1,2徐叶 1,2刘如石 1,3[ ... ]王庆林 1,2
作者单位
摘要
1 湖南省免疫诊断试剂工程研究中心, 长沙410006
2 湖南师范大学医学院, 长沙410006
3 湖南师范大学医学院, 长沙410006,
人巨细胞病毒(HCMV)是全球范围内普遍感染的一种疱疹病毒, 也是引起胎儿先天性畸形和器官移植受者死亡最常见的病原体。有研究表明,gH蛋白是HCMV主要的中和性抗原, 特异性抗gH抗体具有中和HCMV及阻断病毒在细胞间传播的潜力。本研究以含人巨细胞病毒临床株Toledo全基因组的细菌人工染色体(BAC)为模板, 扩增去除信号肽和跨膜区的gH基因片段, 并将其插入表达载体构建pET32a′-gH重组表达质粒, 将序列鉴定正确的重组质粒转化进入表达菌株TransB, 诱导重组蛋白表达。用纯化的重组gH蛋白免疫8周龄BALB/c雄鼠, 经杂交瘤技术获得5株稳定分泌抗gH单克隆抗体的细胞株, 分别命名为8A9、8B4、8C4、8D9、8D12。所获5株单克隆抗体对gH蛋白均具有良好的反应性, 其中8A9、8B4、8D9和8D12具有一定的病毒捕获能力, 且8D9和8D12的捕获能力较强。本研究获得了具有自主知识产权的抗gH单克隆抗体, 抗体具有良好的病毒捕获能力。在此基础上, 我们将进一步鉴定其是否为中和抗体, 为今后开发治疗HCMV感染的中和抗体奠定基础。
人巨细胞病毒 gH糖蛋白 单克隆抗体 病毒捕获 human cytomegalovirus gH protein monoclonal antibody virus capture 
激光生物学报
2020, 29(3): 260
Xin Tian 1,2,3Hao Li 1,2,3Le Liu 1,2,3Meng Wang 1,2,3[ ... ]Zefeng Wang 1,2,3,*
Author Affiliations
Abstract
1 College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, China
2 State Key Laboratory of Pulsed Power Laser Technology, Changsha 410073, China
3 Hunan Provincial Key Laboratory of High Energy Laser Technology, Changsha 410073, China
We report here an ultra-broadband linearly polarized (LP) LP01 LP11 mode converter operating at 1 μm based on a long period fiber grating (LPFG) fabricated in a conventional two-mode fiber (TMF) by a line-focused CO2 laser. The measured 3 dB bandwidth is about 240 nm, which is the broadest bandwidth for such fiber mode converters. The maximum conversion efficiency between the LP01 and LP11 modes is >99% over the range of 1000 nm to 1085 nm, almost covering the whole emission band of Yb3+, which is useful for further power scaling of high-power fiber lasers operating at the 1 μm band.
060.2340 Fiber optics components 060.3735 Fiber Bragg gratings 060.2330 Fiber optics communications 140.3510 Lasers, fiber 
Chinese Optics Letters
2019, 17(12): 120602
作者单位
摘要
长春理工大学电子信息工程学院, 吉林 长春 130022
针对集成成像技术视角窄的问题,提出了一种在集成成像计算重构中增大视角的方法。根据集成成像原理,利用相邻元素图像之间的相似性,对相邻元素图像单元进行配准、拼接,扩大每个微透镜对应的元素图像区域,以减少重构时所提取像素超过元素图像区域的情况发生,从而扩大了计算重构完整3D图像的视角。相比传统方法,本文方法在观看方向偏离光轴较大,超过传统视角一定范围时,元素图像也可以通过对应的微透镜进行正确合成,但由于计算重构时增加了相邻元素图像的配准、拼接过程,因此计算时间有所增加。
图像处理 集成成像 计算重构 视角 元素图像拼接 
光学学报
2019, 39(11): 1110001
作者单位
摘要
1 中南大学湘雅医院神经内科, 湖南 长沙 410006
2 湖南师范大学生命科学学院, 湖南 长沙 410081
gpr98基因突变与多种疾病相关, 该基因在人类和斑马鱼中高度保守。建立斑马鱼gpr98 基因突变体稳定系, 可为阐释 gpr98基因功能提供良好的动物模型和研究基础。本文利用CRISPR/Cas9基因敲除技术在斑马鱼gpr98基因2号外显子上选取两个相距42 bp的靶位点, 分别体外合成sgRNA, 并与Cas9 mRNA一起共注射至斑马鱼胚胎单细胞期的胚胎内。随机挑选发育72 h胚胎提取基因组DNA进行PCR分析, 结果表明: 除了有野生型DNA带外, 部分胚胎有一条比野生型DNA小的带; 进一步将F0代阳性个体与野生型的斑马鱼杂交, 对杂交后代进行基因型分析, 并成功筛选到缺失48 bp(Δ48 bp)的稳定遗传突变的gpr98基因敲除斑马鱼模型。该试验模型的构建为研究gpr98基因在心血管以及骨骼等组织器官的发育及相关疾病发生中的作用奠定了重要基础。
斑马鱼 gpr98基因 zebrafish CRISPR-Cas9 CRISPR-Cas9 gpr98 gene 
激光生物学报
2019, 28(3): 258
刘乐 1王蒙 1奚小明 1,2,3王泽锋 1,2,3,*赵国民 1,2,3
作者单位
摘要
1 国防科技大学前沿交叉学科学院, 湖南 长沙 410073
2 国防科技大学高能激光技术湖南重点实验室, 湖南 长沙 410073
3 国防科技大学大功率光纤激光湖南省协同创新中心, 湖南 长沙 410073
采用二氧化碳激光逐点刻写技术对2 μm波段长周期光纤光栅(LPFG)的传输特性进行了实验研究,探索了光栅周期、折射率调制深度、光栅周期数等光栅刻写参数对光纤光栅在2 μm波段特征损耗峰的影响。仿真和实验结果均表明,2 μm波段LPFG的谐振中心波长和谐振峰深度可分别通过光栅周期以及光栅长度来调谐,激光扫描次数以及折射率调制都将增加光纤内模式的耦合强度。此外还探究了2 μm波段LPFG对于环境温度的敏感特性,通过设计实验测得该波段LPFG的温度灵敏性为74 pm/℃。该研究在2 μm波段光纤激光器及其应用的核心器件方面有潜在的应用价值。
光纤光学 光纤激光 光纤传感 光纤光栅 谐振波长 
光学学报
2019, 39(3): 0306002
Meng Wang 1,2,3Le Liu 1,2,3Zefeng Wang 1,2,3Xiaoming Xi 1,2,3Xiaojun Xu 1,2,3
Author Affiliations
Abstract
1 College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, China
2 State Key Laboratory of Pulsed Power Laser Technology, Changsha 410073, China
3 Hunan Provincial Key Laboratory of High Energy Laser Technology, Changsha 410073, China
The average power of diode-pumped fiber lasers has been developed deeply into the kW regime in the past years. However, stimulated Raman scattering (SRS) is still a major factor limiting the further power scaling. Here, we have demonstrated the mitigation of SRS in kilowatt-level diode-pumped fiber amplifiers using a chirped and tilted fiber Bragg grating (CTFBG) for the first time. The CTFBG is designed and inscribed in large-mode-area (LMA) fibers, matching with the operating wavelength of the fiber amplifier. With the CTFBG inserted between the seed laser and the amplifier stage, an SRS suppression ratio of ${\sim}10~\text{dB}$ is achieved in spectrum at the maximum output laser power of 2.35 kW, and there is no reduction in laser slope efficiency and degradation in beam quality. This work proves the feasibility and practicability of CTFBGs for SRS suppression in high-power fiber lasers, which is very useful for the further power scaling.
fiber Bragg gratings fiber lasers high power stimulated Raman scattering 
High Power Laser Science and Engineering
2019, 7(1): 01000e18
Meng Wang 1,2,3Zefeng Wang 1,2,3,*Le Liu 1,2,3Qihao Hu 1,2,3[ ... ]Xiaojun Xu 1,2,3
Author Affiliations
Abstract
1 College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, China
2 State Key Laboratory of Pulsed Power Laser Technology, Changsha 410073, China
3 Hunan Provincial Key Laboratory of High Energy Laser Technology, Changsha 410073, China
The average power of fiber lasers has been scaled deeply into the kW regime in the past years. However, stimulated Raman scattering (SRS) is still a major factor limiting further power scaling. Here, we have demonstrated for the first time, to the best of our knowledge, the suppression of SRS in a half 10 kW tandem pumping fiber amplifier using chirped and tilted fiber Bragg gratings (CTFBGs). With specially self-designed and manufactured CTFBGs inserted between the seed laser and the amplifier stage, a maximum SRS suppression ratio of >15 dB in spectrum is observed with no reduction in laser efficiency. With one CTFBG, the effective output power is improved to 3.9 kW with a beam quality M2 factor of 1.7 from <3.5 kW with an M2 factor of >2; with two CTFBGs, the effective laser power reaches 4.2 kW with an increasing ratio of 20% and an M2 factor of 1.8, and further power improvement is limited by the power and performance of the 1018 nm pump sources. This work provides an effective SRS suppression method for high-power all-fiber lasers, which is useful for further power scaling of these systems.
Photonics Research
2019, 7(2): 02000167
Author Affiliations
Abstract
1 MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics South China Normal University Guangzhou 510631, P. R. China
2 Department of Ophthalmology Xinhua Hospital, Shanghai Jiaotong University School of Medicine Shanghai 200092, P. R. China
3 Shenzhen Key Laboratory for Minimal Invasive Medical Technologies Graduate School at Shenzhen, Tsinghua University Shenzhen 518055, P. R. China
4 MOE Key Laboratory of Laser Life Science College of Biophotonics, South China Normal University Guangzhou 510631, P. R. China
We proposed a new saccharides sensor developed by symmetrical optical waveguide (SOW)- based surface plasmon resonance (SPR). This unique MgF2/Au/MgF2/Analyte film structure results in longer surface plasmon wave (SPW) propagation lengths and depths, leading to an increment of resolution. In this paper, we managed to decorate the dielectric interface (MgF2 layer) by depositing a thin polydopamine film as surface-adherent that provides a platform for secondary reactions with the probe molecule. 3-Aminophenylboronic acid (3-PBA) is chosen to be the saccharides sense probe molecule in the present work. The aqueous humor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results demonstrated that this sensor shows great potential in monitoring the blood sugar and can be adapted in the field of biological monitoring in the future.
Saccharides sensor surface plasmon resonance symmetrical optical waveguide 3-Aminophenylboronic acid dopamine 
Journal of Innovative Optical Health Sciences
2015, 8(2): 1550003
张怡龙 1,*刘乐 1,2郭峻 1张鹏飞 1[ ... ]何永红 1
作者单位
摘要
1 清华大学深圳研究生院光学检测与成像实验室(深圳市无损监测与微创医学技术重点实验室), 广东 深圳 518055
2 清华大学深圳研究生院绿色化学电源实验室, 广东 深圳 518055
表面等离子体共振(SPR)传感系统有角度谱、光谱、强度、相位等解调方式, 其中光谱型的(SPR)传感系统因可以使用光纤导光, 将传感部分独立出来, 可进行远距离传感和现场检测, 并能有效缩小系统的体积。对称光波导型(SOW)SPR因金属膜层两边的折射率完全相同, 表面等离子体波传播距离更长, 穿透深度更深, 比传统的SPR系统具有更高的灵敏度和分辨率。对对称波导型(SOW)SPR进行光谱解调研究, 以MgF2-Au-MgF2结构的SOW-SPR为传感单元, 同时以光纤输出的卤素灯为光源, 搭建了一套光谱解调的SOW-SPR检测系统, 以不同浓度的葡萄糖溶液对系统折射率分辨率进行测量, 得到2.8×10-7 RIU的分辨率。为SOW-SPR系统小型化、现场检测以及远距离探测提出一种可能实现的手段, 具有很好的应用前景。
表面等离子体共振 光谱解调 对称光波导 Surface plasmon resonance Spectral interrogation Symmetrical optical waveguide 
光谱学与光谱分析
2015, 35(2): 289

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